A New Species of Ampelovirus Detected in Persea lingue (Ruiz & Pav.) Nees ex Kopp, a Common Tree of the Threatened Chilean Sclerophyll Forest

Biotic and abiotic stress seriously affects the development of plants, leading to death a significant number plants in natural landscapes. Over last twelve years, central zone Chile has been under an intensive drought, affecting species inhabiting Chilean Mediterranean forest, which is classified as biological hotspot. In this context, our group started small survey monitor presence intracellular pathogens that may be infecting increasing damage caused by water deprivation. Several one species, Persea lingue, showed symptoms interveinal yellowing leaf curling. RNA-seq analyses these samples long contig with high coverage only symptomatic plants. Phylogenetic analysis molecular features indicated it was new Ampelovirus genus. RT-PCR determined virus same preserve.